MARíA O. LONGAS                                                        

                                       CURRICULUM VITAE

Purdue University Calumet, Dept. of Chemistry and Physics.  2200 169TH Street, Hammond, IN 46323-2094. Phone: 219–989–2286. Fax: 219–989–2130.  E-mail: mol@calumet.purdue.edu

                                           1 - Education

1978, Oct.      Ph. D., Chemistry, New York University

Ph. D. Thesis: "Effect of Dithiothreitol on the Thrombin Antithrombin III Reaction and the Covalent Nature of the Antithrombin III–Thrombin Bond." Using radioactive isotopes, chemical and biochemical techniques, I demonstrated that the reaction of thrombin with its natural inhibitor, Antithrombin III, yielded a carboxylic ester between the two proteins. This ester has been confirmed by other workers, using X–ray crystallography.

After obtaining an MA in chemistry, I had a full time position as a research assistant at New York University Medical Center (1974–1978), while working on my Ph. D. at night and over the weekends. During this time, I took one graduate course to maintain student status. Thesis advisor: Thomas H. Finlay.

1973   MA      Organic Chemistry, New York University

1971   BA       Chemistry,      Hunter College, City University of New York

                                        2 – Additional Training AND EXPERTISE

A. Methodology

  1. Varian, Nuclear Magnetic Resonance Spectrometer use (300, 500 and 600 MHz), The University of Notre Dame, Dr. Anthony Serianni’s Laboratory, Fall 2003 (sabbatical leave from Purdue University Calumet)
  2. Pt catalyzed sugar oxidations, The University of Notre Dame, Dr. Anthony Serianni’s laboratory, Fall 2003 (sabbatical leave)
  3. Microwave Enhanced Sample Preparation: General and Environmental Applications –sponsored by the American Chemical Society, Pittsburgh, PA, May 11–15, 1998
  4. Analytical Fourier Transform Infrared Spectroscopy Workshop: Techniques, Applications, Computer Methods –sponsored by the American Chemical Society, Atlanta, GA, March 15–17, 1997
  5. Learn New Applications in Mass Spectrometry Workshop –sponsored by Hewlett Packard, Naperville, IL, June 10, 1996
  6. Advanced Nuclear Magnetic Resonance Spectroscopy Workshop –sponsored by the National Science Foundation, Rhode Island University, July 30–August 8, 1996
  7. Nuclear Magnetic Resonance Spectroscopy of Carbohydrates and Proteins, a 10-month training in the laboratory of Dr. Hans Vliegenthart, Utrecht University, Dept. of Bioorganic Chemistry, Utrecht, the Netherlands (sabbatical leave from Purdue University Calumet, 1993–1994)
  8. Gas Chromatography/Mass Spectrometry (GC/MS) of Methylated and Acetylated Sugars. Utrecht University, Dept. of Bioorganic Chemistry, Feb. through April 1994
  9. Solid Extraction Workshop –sponsored by Jones Chromatography and International Sorbent Technology, Chicago, IL, July 1994
  10. Fourier Transform Infrared Spectroscopy Workshop –sponsored by Spectra-Tech Seminars Midwest, Inland Meeting Center, Westmont, Chicago, IL, June 1989
  11. Recombinant DNA Technology Workshop -sponsored by Chataqua Courses for College Teachers. Temple University, April 1988
  12. Biology of Human Skin Aging –one week continuing education course for physicians and scientists sponsored by the National Institute on Aging (NIA), Jefferson College, May 1988

B. Special Expertise

Bimolecular Purification: I have extensive experience in the purification of proteins and glycosaminoglycans from biological tissues, using ion exchange, gel permeation and affinity chromatography; in addition to fractional precipitation and liquid-extraction, methods, among others.

   Analytical: Fourier transform (FT)–nuclear magnetic resonance spectrometry, FT–Infrared/Raman spectroscopy, gas chromatography, mass spectrometry, high performance liquid chromatography (HPLC), scintillation counting, amino acid analysis, using thin layer chromatography, HPLC or the amino acid analyzer, gel electrophoresis and gel electro-elution. I am also familiar with proteolytic and glycosidase enzymes of normal and pathologic human tissues.

    Tissue Culture: Human skin fibroblasts in culture are used in my laboratory as the source of isotopically labeled glycosaminoglycans.

                                         3 – ACADEMIC APPOINTMENTS

·        2003-2004 - Research Scholar, University of Notre Dame, Department of Chemistry and Biochemistry, South Bend, IN 46637 (sabbatical leave from Purdue University Calumet)

·         1997–2001, Coordinator of Chemistry and Assessment, Purdue University Calumet, Hammond, IN (equivalent to Co-Chair)

·         1/02/95–6/30/95, Acting Dept. Head, Chemistry & Physics, Purdue University Calumet, Hammond, IN

·         1990 –1996, Associate Professor of Chemistry, Purdue University Calumet, Hammond, IN

·         1987–1990, Assistant Professor of Chemistry, Purdue University Calumet, Hammond, IN

·         1983–1987, Assistant Professor, The Mount Sinai School of Medicine, Dept. of Dermatology, New York, NY

                                           4 – PROFESSIONAL HISTORY

                                           5 – Research Interests

                 6 – COMMITTEES, Boards and Other Offices

I. University Committees

·        Fall 2006, Area Promotions and Tenure Committee

·        Fall 2006, METS Promotion & Tenure Committee

·        Fall 2005, Primary Promotions Committee, Department of Computer Instruction Technology (CIT)

·        Fall 2005, Area Promotions and Tenure Committee, School of Technology

·        2003-2005, Library Committee (except during sabbatical leave, 2003)

·        2004-2005, Purdue University Calumet Senate and its Educational Policy Committee

·        2004, Primary Promotions Committee, Dept. of Biological Science.

·        2002–2003, Grade Appeals Committee. Area Promotions and Tenure Committee, School of Technology. ISCP Department Head Search Committee

·        2001–2003, Purdue University Calumet Senate and its Educational Policy Committee

·        1997–2003, 2005, Area Promotion Committee, School of Engineering Mathematics and Science (EMS)

·        2001­2002, Primary Promotions Committee, Dept. of Biological Science.

·        2001, EMS Strategic Planning Committee

·        2000–2001, Primary Promotions Committee, Department of English and Philosophy

·        2000–2001, Outstanding Service Award Committee (chair).

·        1998–2001, Women Studies Advisory Committee

·        1997–1999, Primary Promotions Committee, Department of Electrical Engineering Technology

·        1997, EMS Vision Committee

·        1996–1997, Committee on Academic Administrative Organization.

·        1991–1994/97, Outstanding Teacher Award Committee

·        1995–1996, Mentor for the Summer Resident McNair Program. This involved one on one training of the students in residence, who were supposed to work on their research projects 30 hr per week. They also had to be coached for the semiprofessional presentation of their results at a seminar held on the 8th week of the internship

·        1996, Outstanding Research Award Committee

·        1992–1993, McNair Faculty Advisory Council; and Engineering & Science Pre-College Program Steering Committee

·        1990–1993, Advisory Committee on Grants and Proposals

·        1991–1992, Council of the School of General Studies

·        1989–1992, Council of Faculty Delegates. Council Nominating Committee and the chair of the Research and Educational Policy Committee

·        1988–1992, Faculty Development Committee

·        1990, Chancellor Search and Screen Committee

·        1988–1989, Committee on Celebrating Scholarship

II. Committees of the Department of Chemistry and Physics

·        Fall 2006, Primary Promotions Committee

·        2005-2006 Retention Committee

·        2000–2005, Primary Tenure Committee (except during in 2003, sabbatical leave)

·        2001–2002, Curriculum Committee (chair)

·        1988–2002, Faculty Search Advisory Committee (chair, several times)

·        2000–2001, Primary Promotions, Chemistry Outreach, and Chemistry Assessment Committees 

·        1997–1999, Equipment, Undergraduate Planning, and Tenure Committees

·        1989/93/94–99, Promotions and Tenure Advisory/Elections Referendum Nomination and Tellers Committee

·        1997–1998, Laboratory Safety Committee

·        1991, 1992 (chair), 1993 and 1995–1998, Information and Resources Committee

·        1992 (chair) /93/95/97–Intradepartmental Services & Facilities Advisory Committee

·        1995–1997, General Chemistry Laboratory Coordinator

·        1994, Assessment Committee

III. Community Service

·        “Biochemistry: the Link Between Biology and Chemistry” – PUC, Dept. of Biological Sciences,  March 31, 2006

·        EMS Open House, Chemistry Demonstrations, November 2006

·        Celebrating Diversity of Scholarship (Presenter).  PUC, April 17, 2006

·        Career Day (chemistry demonstrations), Gary Community School Corporation, May 17, 2006.

·        Calumet Regional Science Fair (judge), 1989-1993 and 1995-2007

·        Career Day, Munster High School, May 4, 2005

·        Majors Fair (participant), Purdue University Calumet, 1997 and 1998 – 2003

·        Career Day (chemistry demonstrations), Gary Community School Corporation, May, 1997 and March 1998

·        Workplace Gender Equity Project (speaker), Purdue University Calumet, 1997 1998

·        Day on Campus (speaker), 1995 -1996

·        Science Olympiad Workshop (teacher), 1994

·        Calumet Regional Science Olympiad (judge), 1991-1994

·        Advanced Engineering and Science Summer Program (teacher), July 1992

·        Engineering and Science Summer Program for seventh–, eight– and ninth–grade students, Purdue University Calumet, June 17 –July 19, 1991 and June 15 –July 17, 1992 (organizer and teacher)

·        Advisory Committee of Senator Roy M. Goodman (member), New York, 1983-1987

·        The Community Church Shelter for Homeless Men (founder and director), New York City, January 1983 –July 1987

·        Safety Network", the Newsletter of the Coalition for the Homeless, New York City, 1982 –1984 (co-editor)

IV. Miscellaneous

·         1988–University Booth at Waterfront Festival, participant, Purdue University Calumet

                                          7 – PROFESSIONAL SOCIETIES

·         1991–The present, Society for Applied Spectroscopy

·         1986–The present, Union of Concerned Scientists

·         1981–The present, International Union of Pure and Applied Chemistry.

·         1981–1986, Society for Complex Carbohydrates*

                                     8 – HONORS

·        1998–The present, The Academy of American Poets (member).

·        1988–2006, Scholarly Research Release Award from Purdue University Calumet; a 0.25 full time equivalent (FTE) per semester

·        1971–The present, Iota Sigma Pi, National Honor Society for Women in Chemistry–Hunter College (member)

·        1991, Outstanding Teacher-Student Biology Club (Purdue University Calumet)

·        1990, Outstanding College Teacher-Purdue University Calumet

·        1987, Dynamic Woman of the 80's-Unitarian Universalist Women Association of New York Metro District

·        1985 & 1987, Consultant-National Institutes of Health

·        1984, Visiting Scientist-Dept. of Oral Biology, University of Alberta, Edmonton, Canada, Dec. 10–14

·        1971, the Emma Wagner Ackerle Scholarship Award for the Exceptionally Gifted Student–Hunter College, CUNY

·        1971, BA Cum Laude–Hunter College, CUNY

                                          9 – Biographical Citations

·        2004-2005, The Manchester Who’s Who Registry

·        1992-2003, WHO is Who in Science and Engineering (Marquis)

·        1994-2003, WHO is Who in the Midwest

·        1991-2003, WHO's Who of American Women

·        1997. The World Who is Who of Women – 13th Ed.

                                        10 – BOOK Acknowledgments

Chemistry and Life: An Introduction to General, Organic, and Biological Chemistry, 4th edition (1993) by J. W. Hill, D. M. Feigl and S. J. Baum. Prentice Hall.

                                               11 – INVITED Lectures

1.      hyaluronic Acid, the Molecule of Youth is Severely Affected by Aging −Purdue University Calumet, Dept. of Biological Sciences – February 8, 2006

2.      hyaluronic Acid, the Molecule of Youth is Severely Affected by Aging, Discovery Park, Purdue University West Lafayette, November 23, 2005

3.      Changes in Hyaluronic Acid Composition in Human Skin: The Effect of Aging −Dept. of Biological Sciences, Purdue University Calumet, February 25, 2005

4.      Bringing Research to the Students −Key note speaker at NSF Sponsored Conference on Undergraduate Research, University of Northern Iowa (UNI), Cedar Falls, IA,  July 30, 2004

5.      Novel Approach to Sequencing Glycosaminoglycans The University of Notre Dame, Dept. of Chemistry and Biochemistry, September 2003

6.      Life as a Chemist, “Gender Equity”, Purdue University Calumet (4/17/98)

7.      One Day in the Life of a Woman Chemist, “Gender Equity”, Purdue University Calumet (4/15/97)

8.      Effect of UV Light Irradiation on Skin Glycosaminoglycans, Purdue University Calumet, Dept. of Chemistry and Physics (2/28/95)

9.      Reasons for Studying Skin Glycosaminoglycans, Loyola University of Chicago, Dept. of Chemistry (10/27/94)

10. A Reason for Studying Skin Glycosaminoglycans, Plastic Surgeons of St. Margaret Mercy Health Care Center, Hammond, IN. (7/28/94)

11. The Protein Linkage Region of Human Skin Dermatan Sulfate. Report II Bijvoet Center for Biomolecular Research, Dept. of Bioorganic Chemistry, Utrecht, the Netherlands (6/01/94)

12. The Protein Linkage Region of Human Skin Dermatan Sulfate. Report Bijvoet Center for Biomolecular Research, Dept. of Bioorganic Chemistry, Utrecht, the Netherlands (3/30/94)

13. Dermatan Sulfate in Aging and Disease Bijvoet Center for Biomolecular Research, Dept. of Bioorganic Chemistry, Utrecht, the Netherlands (11/19/93)

14. Is Dermatan Sulfate of Human Skin Bonded to Two Different Amino Acids on the Core Protein, Bijvoet Center for Biomolecular Research, Dept. of Bioorganic Chemistry, Utrecht, the Netherlands (9/22/93)

15. Search for the Role of Dermatan Sulfate in Aging and Disease, Chicago College of Osteopathic Medicine, Downers Grove, IL (4/09/93)

16. The Social Impact of the Physical Sciences, Purdue University Calumet, Dept. of Biology, SCI 300 (9/28/92)

17. Derma tan Sulfate. Does It Play a Role on Skin Pathology, Indiana University Fort Wayne Center for Medical Education, Fort Wayne, IN (7/24/92)

18. Could Vitamin E Protect Skin From UVB-Light Damage? Lark High School, Hammond, IN (12/11/91)

19. Biochemistry of Human Skin Aging, East Chicago Central High School, East Chicago, IN (4/26/90)

20. What is Retin A: Does It Reverse Aging?, Purdue University Calumet, Geriatric Center (3/19/89)

21. Effect of Age on Human Skin, the Methodist Hospital, North-Lake Campus, Gary, IN (6/22/88)

22. A Biochemical View of the Aging of Skin: Scientific Understanding and Prevention Purdue University Calumet Chapter, Student Affiliates of the American Chemical Society, Hammond, IN (3/23/88)

23. Aging of Human Skin, Dept. of Biology, Purdue University Calumet, Hammond, IN (2/19/88)

24. I Was a Stranger and You Sheltered Me, Unitarian Universalist Women's Association of the New York Metro District, New York, NY (1/24/87)

25. Glycosaminoglycans and Pseudoxanthoma Elasticum, First Natl. Symposium on Pseudoxanthoma Elasticum. The Mount Sinai School of Medicine, New York, NY (1/07/87)

26. Alterations of Hyaluronic Acid and Dermatan Sulfate in Aging Human Skin, Indiana University Northwest Center for Medical Education, Gary, IN (4/21/86)

27. Human Skin and Aging, Geriatrics Dept., The Mount Sinai School of Medicine, New York, NY (11/05/85)

28. The Aging Human Skin. Dermatology Grand Rounds, the New Jersey School of Medicine and Dentistry, Newark, NJ (10/07/85)

29. Proteodermatan Sulfate, the Proteoglycan of Mammalian Skin, University of Alberta, Dept. of Oral Biology, Edmonton, Canada (12/11/84)

30. Ethics in the Professions, The Community Church of New York, New York, NY (10/14/84)

31. Human Skin Glycosaminoglycans: Structure, Functions, Metabolism, Methods for Identification in Intact Skin, Involvement in Skin Disorders, The Mount Sinai School of Medicine, New York, NY (3/28/83)

32. Current Methods for Protein Purification from Biological Fluids and Tissues, The Mount Sinai School of Medicine, New York NY, Nov. 1981

12 – Gordon and other research conferences

                                    Attended

·        Gordon Research Fonference on Carbohydrates, Tilton, NH, June 19-24, 2005

·        Gordon Research Conference on Proteoglycans, Proctor Academy, Andover, NH, July 11-16, 2004

·        Gordon Research Conference on Carbohydrates, Tilton, NH, June 2003

·        Gordon Research Conference on Proteoglycans, Proctor Academy, Andover, NH, July 2002

·        Gordon Research Conference on Polysaccharide Chemistry, Mt. Holyoke College, South Hadley, Mass., July 2001

·        Gordon Research Conference on Carbohydrate Chemistry, Tilton School, Tilton, NH, June 2001

·        Mechanisms and Consequences of Aging, Purdue University, West Lafayette, April 2001

·        Cyclodextrins, American Chemical Society, Chicago Section, Cerestar, Hammond, April 2001

·        Laboratory Productivity Seminar, Chicago State University, Chicago, IL, May 2001

·        Midwest Glycoscience Group Seminar, Dionex Corporation, Westmont, IL, May 1996

·        Gordon Research Conference on Glycobiology, Casa Sirena, Oxnard, CA, January 1995

·        Gordon Research Conference on Proteoglycans, Proctor Academy, Proctor, NH, June 1990

·        Gordon Research Conference on Proteoglycans, Proctor Academy, Proctor, NH, June 1986

·        Gordon Research Conference on Preteoglycans, Plymouth State College (N), Plymouth, NH, June 1984

                                 13 – Referee for Scientific Journals

·        The FASEB JOURNAL (2005

·        Biomacromolecules (2004, 2006). 

·        Carbohydrate Research (2000-2004).

·        Analytical Biochemistry (1990-1992.

·        Journal of Gerontology (1988-1990).

·        Journal of Investigative Dermatology (1986-1990).

                                14  GRANTS and Awards Received

1.      Purdue University Calumet, Academic Affairs Summer Faculty Grant. Title: Purification of Hyaluronan N-deacetylase From Human Skin. Role: Principal Investigator. $ 6,000.00, summer 2004.

2.      Purdue University Calumet, Dept. of Chemistry and Physics, Faculty Development, Summer Faculty Grant. Title: The N-deacetylase of Aging Human skin. Role: Principal Investigator. $ 6,000. Summer 2001.

3.      FILIP DUE 9650826 Grant from the National Science Foundation. Title: “Incorporating FT–Infrared into the Laboratory Curriculum.” Co-principal investigators: Charles E. Kriley, María O. Longas and Michael W. Pelter. Direct cost: $ 23,308.20. Period: 8/01/95–1/31/97.

4.      Travel Grant–Purdue Research Foundation, 1989 ($ 1000), 1992 ($ 1140) and 1993 ($1,060).

5.      Autodesk Educational Grant for the Purchase of Hyperchem 3.0 –PUC, 1993.

6.      XL–Summer Faculty Grant–Purdue Research Foundation, 1988 ($ 3900), 1990 ($ 5000) and 1991 ($ 5000).

7.      RO1–AGO3877 Grant from the National Institute on Aging. Title: "Hyaluronic Acid and Dermatan Sulfate in Aging Human Skin."  Principal Investigator: María O. Longas. Direct cost $ 210,000. Period: 9/29/83 to 2/28/87. Indirect cost: 65% of the direct cost.

                                         15 – TEACHING EXPERIENCE

I. At Purdue University Calumet

a) Courses Taught

·        CHM 499 B, CHM 333L. Instrumental Biochemistry, a new course for Biology Majors (Spring 2003)

·        CHM 111, General Chemistry (lecture and laboratory)

·        CHM 115, General Chemistry for chem. majors (lecture and laboratory).

·        CHM 116, General Chemistry for chem. majors (lecture and laboratory).

·        CHM 119, General Chemistry for Nursing Students (lecture and laboratory, 1987 through 1993 and Fall 2005)

·        CHT 202, Individual Projects in Chemical Technology

·        CHM 224, Analytical Chemistry (laboratory)

·        CHM 254, Organic Chemistry (laboratory)

·        CHM 255 & 256 Organic Chemistry I & II (lecture)

·        CHM 290, Special Topics in Chemistry (Research in Chemistry)

·        CHM 318, Biomolecular NMR Spectroscopy/Magnetic Resonance Imaging (lecture & laboratory)

·        CHM 319, Introduction to Biochemistry for Nursing Students (lecture and laboratory)

·        CHM 320, Analytical Techniques in Biochemistry (lecture and laboratory). CHM 333, Principles of Biochemistry (lecture)

·        CHM 499, Special Topics in Biochemistry (Research in Biochemistry)

·        CHM 533, Biochemistry (graduate level lecture)

·        CHM 534, Biochemistry (graduate level lecture)

·        CHM 599A, Graduate Level Biochemistry Research

b) Undergraduate and Graduate Students Trained on Individual Projects in Chemistry 290, 320, 499, 599 and Chemical Technology (CHT) 202. The names of the students who did publishable work appear as coauthors in the publications.

·        Spring 2007, Steven Dubec

·        Fall 2006, Steven Dubec

·        Summer 2006, Steven Dubec, Christopher Pardo (Biol. Graduate Student)

·        Spring 2006, Bertha Salazar, Anna Dunning

·        Fall 2005, Olutobi Tele-Ekundayo, Tracy Stahl.

·        Spring 2005, William Xie, graduate student, Dept. of Biological Sciences, Purdue Univeristy Calumet

·        Spring 2004, Michelle Puchalski

·        Fall 2003, Kenya Cheairs and Michelle Puchalski

·        Spring and Summer 2003: Kenya Cheairs and Michelle Puchalski

·        Fall 2002: Kenya Cheairs and Christopher Dmytriw

·        Summer 2002: Kenya Cheairs

·        Spring 2001: Victoria Eulloqui

·        Spring & Fall 2000: John Lesniak

·        Fall 1999: John Lesniak and Jennifer Trinkle

·        Summer 1999: John Lesniak, Brenda McKinstry and Jennifer Trinkle

·        Spring 1999: John Lesniak, Brenda McKinstry and Jennifer Trinkle

·        Spring 1998: Ryan Booth, James McPencow (biology graduate student) and Jennifer Trinkle

·        Summer 1998: Ryan Booth, James McPencow, Brenda McKinstry and Jennifer Trinkle

·        Fall 1998: Ryan Booth, Brenda McKinstry and Jennifer Trinkle

·        Spring 1997: Timothy Frankovich (CHT 202) and Brian Harmon

·        Summer 1997: Elizabeth Kenyon and Jennifer Trinkle

·        Fall 1997: Ryan Booth, James McPencow, Kevin Thacker (CHT 202) and Jennifer Trinkle

·        Spring 1996: James Burden, Joe Clemons, Susan Oehlman and Christopher D. Schoeneck

·        Summer 1996: Joe Clemons, Brian Kuzera, Susan Oehlman, Mary A. Sap (CHT 202) and Christopher D. Schoeneck

·        Fall 1996: Joe Clemons and Christopher Schoeneck, Brian Harmon.

·        Spring 1995: Brian Harmon

·        Summer 1995: Brian Harmon, Gregory Hughes and Taiye Hattisburg.

·        Fall 1995: James Burden, Brian Harmon and Christopher Schoeneck.

·        Fall 1994: Brian Harmon and Christine McBride

·        Spring 1992: Mary Ramos, Stacia Sorrell (biology graduate student) and Radhakrishnamurty Vemulapalli (M. D.)

·        Spring 1991: Kathy Companik, Anne Dukehart, Robert Schwartz, Stacia Sorrell (biology graduate student), and Mary Tran

·        Summer 1991: Kathy Companik, Stacia Sorrell, Carol Vander–Lugt

·        Fall 1991: Mary Ramos and Stacia Sorrell

·        Spring 1990: Christy Beck (biology graduate student), Karl O. Breitweiser, Michelle Brown, and Debbie Pruiett

·        Summer 1990: James Alb, Robert Schwartz, Stacia Sorrell and Mary Tran

·        Fall 1990: Lorraine Celis, Anne Dukehart, Robert Schwartz, Stacia Sorrell and Mary Tran

·        Spring 1989: Karl O. Breitweiser, Charles Muncrief and Samuel McPheron

·        Summer 1989: Christy Beck (biology graduate student), Michelle Brown, Karl O. Breitweiser, Debbie Pruiett, Samuel McPheron and Charles Muncrief

·        Fall 1989: Christy Beck (biology graduate student), Michelle Brown, Karl O. Breitweiser, Debbie Pruiett, Charles Muncrief and Daniel Vaughn (CHM 202)

·        Fall 1988: Karl O. Breitweiser

·        Spring/Summer 1988: Christine Gutsch

c) Courses Developed

CHM 333L, Analytical Biochemistry. The purpose is to train biology majors and chemistry minors in analytical, biochemical techniques utilizing modern equipment. The chemistry majors are exposed to this equipment in Instrumental Chemistry, and follow a different approach. This course was offered for the 1st time in Spring 2003 as CHM 499B, because the official number was not in place then.

CHM 318, Biomolecular NMR Spectroscopy/ Magnetic Resonance Imaging, a new course at Purdue University. It includes multidimensional 1H and 13C- NMR spectroscopy.

CHM 320, Analytical Techniques in Biochemistry, a new course at Purdue University. This course was completely different from CHM 333L described above. It involved biochemical techniques like, thin layer chromatography, 1 and 2d–electrophoresis, gel exclusion chromatography, etc. It was intended for nursing and biotechnology students.

CHM 333, Principles of Biochemistry. This survey course offered at Purdue West Lafayette was restructured for Purdue University Calumet students. These students take the National American Chemical Society exam at the end of the course and score 15 to 25 points above the national average.

d) Courses Modified

The following modifications were introduced to emphasize clarity and relevance.

    CHM 119 –Incorporation of 12–in–class demonstrations including chemical reactions to explain chemical, biochemical and physical phenomena.

    CHM 319 –To show correlation between biochemistry and human disease, students were required to give short, oral presentations on the medical application's) of the experiments they were bout to perform.

    CHM 533 –Students are trained to write 5–page (single spaced) grant proposals on biochemical problems. They defend their proposals in 10-min oral presentations given to the entire class.

    CHM 534 –Students are required to give 35–min seminars on biochemical problems. The purpose is to expose the students to areas of biochemistry available for research, to teach them critical analysis of existent data, and the preparation for basic research in science.

    CHM 290 & 499 –For these courses, I design experiments according to the ability, time and experience of each student.

e) Prospects for Future Development as a Teacher and Statement of Teaching Philosophy

The improvement of my teaching method, which changes according to the group of students, is my constant endeavor. This means that I learn the newest pedagogical techniques and bring them to the classroom. The computer is a useful tool in the presentation of scientific material. Computer programs are used in my lectures as long as they help make the material more comprehensible to the students. The students are also encouraged to use the computer for their research, presentations and writing.

    One way to learn a topic is by explaining it in writing, whether in prose or verse. Hence, my students are required to integrate science and the humanities by writing scientific phenomena in prose or verse. They are also encouraged to present their writings in play form to the entire class.

    My teaching philosophy: No subject is beyond comprehension when presented the right way. Therefore, using simple language to explain complex scientific phenomena with as many visual aids as possible, I try to bring the students to a level of understanding before introducing the scientific terms. Once they understand the topic, I gradually introduce the scientific terms. In other words, I start at the student level, and gradually introduce the teacher level. At the end of a description, I ask the students to recapitulate in their own words what they have just heard, in an attempt to encourage class participation.

    I believe that the best teachers are those who educate themselves continuously, deliver lectures well, allow student participation in class, offer the students unlimited help outside of the classroom, encourage them to work hard, and are concerned with their academic success.

II. At Other Institutions

The Mount Sinai School of Medicine

Invited lectures on the chemistry and biochemistry of mammalian proteoglycans. Advanced Cell Biology, Graduate Level, 1983 and 1984. Course Director, Dr. Saul Puszkin. New York University.

New York University

General and Organic Chemistry Laboratories. Washington Sq. College, New York, NY (1971–1973).

                                           16 - BIBLIOGRAPHY

I–Refereed Papers

1.      Longas*, M. O., +Burden, J. D., +Lesniak, J., +Booth, R., +McPencow, J. A., and Park, J. I.: Hyaluronic Acid N–deacetylase Assay in Whole Skin, Biomacromolecules (2003), 4:189-192.

2.      Longas*, M. O., Garg, H. G., +Trinkle–Pereira, J. A., and Hales, C. H.: Heparin Antiproliferative Activity on Bovine Pulmonary Artery Smooth Muscle Cells Requires Both N–Acetylation and N–Sulfonation, Carbohydr. Res. (2003), 338:251-256.

3.      Longas*, M. O., Schoeneck, C. D., +Trinkle, J. A., Park, J. I. and Griep, J. A.: Human Skin Dermatan Sulfate with Sulfated and Unsulfated Nonreducing Ends, Carbohydr. Res. (1999), 321:261-266.

4.      Lebwohl*, M., Longas, M. O., Konstadt, J., Koreen, R., Grabowski, G. A., Phelps, R., Lemlich, G., Schwartz, E.: Hyaluronic Acid and Dermatan Sulfate in Lesional and Non–Lesional Pseudoxanthoma Elasticum Skin, Clin. Chimica Acta (1995), 238:101-107.

5.      Longas*, M. O., Bhuyan, D. K. Bhuyan, K. C., +Gutsch, C. M. and +Breitweiser, K. O.: Dietary Vitamin E Reverses the Effect of Ultraviolet Light on Rat Skin Glycosaminoglycans, Biochim. et Biophys. Acta (1993), 1156:239-244.

6.      Longas*, M. O. and Garg. H. G.: Sulfate Composition of Dermatan Sulfate from Scar Tissue, Carbohydr. Res. (1992), 237:319-324.

7.      Longas*, M. O. and +Breitweiser, K. O.: Sulfate Composition of Glycosaminoglycans Determined by Infrared Spectroscopy (1991), Anal. Biochem., 192:193–196.

8.      Longas, M. O.: Partial Characterization of Dermatan Sulfate by Proton NMR Spectroscopy (1990), Anal. Biochem., 187:355-358.

9.      Longas*, M. O., Russell, C. S. and He, X–Y.: Evidence for Structural Changes in Hyaluronic Acid and Dermatan Sulfate with Aging (1987), Carbohydr. Res., 159:127–136.

10. Longas*, M. O., Russell, C. S. and He, X–Y.: Chemical Alterations of Hyaluronic Acid and Dermatan Sulfate Detected in Aging Human Skin by Infrared Spectroscopy (1986), Biochim. Biophys. Acta, 884:265-269.

11. Longas*, M. O., Wisch, P., Lebwohl, M., and Fleischmajer, R.: Skin and Urine Glycosaminoglycans in Pseudoxanthoma Elasticum (1986), Clin. Chim. Acta, 155:277-236.

12. Longas*, M. O. and Fleischmajer, R.: Immunoelectron Microscopy of Proteodermatan Sulfate in Human Mid Dermis (1985), Conn. Tissue Res., 13:117–125.

13. Longas, M. O. and Meyer*, K.: Evidence that a Reducible Xylosyl-Lysine Is the Protein Linkage of Dermatan Sulfate (1982), Proc. Natl. Acad. Sci., USA, 79:6225–6228.

14. Longas*, M. O. and Poh-Fitzpatrick, M.: A Tightly Bound Protein-Porphyrin Complex Isolated from the Plasma of a Patient with Variegate Porphyria (1982), Clin. Chim. Acta, 118:218–228.

15. Longas, M. O. and Meyer*, K.: Sequential Hydrolysis of Hyaluronate by b–N–Acetylhexosaminidase and b–Glucuronidase (1981), Biochem. J., 197:275–282.

16. Longas, M. O. and Finlay*, T. H.: The Covalent Nature of the Antithrombin–Thrombin Bond (1980), Biochem. J., 189:481– 489.

17. Longas, M. O. and Poh–Fitzpatrick*, M.: High Performance Liquid Chromatography of Plasma Free Acid Porphyrins (1980), Anal. Biochem., 104:268–276.

18. Longas, M. O. and Finlay*, T. H.: Inhibition of Human Thrombin by Methoxylamine Hydrochloride (1980), Intl. J. Biochem., 11:565–567.

19. Longas, M. O., Newman*, J. and Johnson, A. J.: An Improved Method for the Purification of Human Fibrinogen (1980), Intl. J. Biochem., 11:559–564.

20. Longas, M. O., Ferguson, W. S. and Finlay*, T. H.: Studies on the Interaction of Heparin with Thrombin and Other Plasma Proteins (1980), Arch. Biochem. Biophys, 200:595–602.

21. Longas, M. O., Ferguson, W. S. and Finlay*, T. H.: A Disulfide Bond in Antithrombin Is Required for Heparin–Accelerated Thrombin Inactivation (1980), J. Biol. Chem., 255:3436-3441.

+PUC Student, *senior author.

II-Invited Review Articles (Book Chapters)

1.      Longas, MO: Hyaluronan in Aging. In: Chemistry and Biology of Hyaluronan. Garg, HG and Hales CA, Eds. Amsterdam: Elsevier Science (2004).

2.      Perlish*, J. S., Longas, M. O. and Fleischmajer, R. The Role of Glycosaminoglycans in Aging of the Skin. In: Aging and the Skin, Balin, AK. and Kligman, A., Eds. New York: Raven Press (1989).

 

 

III–Books

·         Castrillón–Longas, María “Reminiscencias” -A book of classical Spanish poems that I have written as a hobby. Matices Publications, Medellín, Colombia (ISBN 958–33–0587–1) (1997)

IV-Abstracts of Work Presented at Scientific Meetings and Published in Refereed Journals

1.      Longas, M. O., Oehlman, Susan, Trinkle Pereira, Jennifer A.: Evidence of Exo-Sulfatase Activity in Quail Egg White (2006) Glycobiology, 16, 11:1125.

2.      Longas, M. O., Banerjee, D. and Xie, W.: Tunicamycin-Induced Apoptosis is Characterized by Protein Denaturation: An Infrared Spectroscopy Study (2005), Glycobiology, 15, 11:1204.

3.      Martinez, J. A., Tavarez, J. J., Oliveira, C. M., Sanchez, A., Baksi, K, Longas, M. O., Banerjee, D. K.: Differential Expression of Cytoplasmic Chaperones (HSP-70 & HSP-90) and ER Chaperons (GRPL-74/Bip & GRP-94) is a key to Tunicamycin-indiced  Unfolded Protein Response-Mediated  Apoptosis in Capillary Endothelial Cells (2005), Glycoconjugate J., 22, 4-5-6:227.

4.      Longas, M. O. Cheairs, K., Puchalski, M. L., Park, J.I.: Fourier Transform Infrared Spectroscopy in the Characterization of Skin (2004), Glycobiology, 14, 11: 1174.

5.      Longas*, M. O. +Puchalski, M. L., +Cheairs, K., Park, J. I.: Glycosaminoglycan Raman Spectroscopy Bands, the Fingerprints of Aging (2003), Glycobiology, 13, 11:888.

6.      Longas*, M. O., +Cheairs, K., +Puchalski, M. L., Park, J. I.: Molecular and Concentration Changes in Whole Skin Glycosaminoglycans Detected by Infrared Spectroscopy (2003), Glycobiology, 13, 11:888.

7.      Longas*, M. O., Garg, H. G., +Trinkle–Pereira, J. A., and Hales, C. A.: Heparin Antiproliferative Activity on Bovine Pulmonary Artery Smooth Muscle Cells Requires Both N–Acetylation and N–Sulfonation (2002), Glycobiology, 12, 10:665.

8.      Longas*, M. O. and Stone, A. L.: Structure-Function Relations of Heparin-Mimetic Sulfated Oligosaccharides: Study of Alternate Chair Conformations by NMR Spectroscopy (2001), Glycobiology, 11, 10:921.

9.      Longas*, M. O. and +Lesniak, J.: Specificity of an Age-Induced N-Deacetylase (2001), Glycobiology, 11, 10:862.

10. Longas*, M. O., +Trinkle, J., Garg, H.G., Hales, C. A.: Heparin N-Acetylation Affects Its Antiproliferative Activity (2000), Glycobiology, 10, 10:1107.

11. Stone*, A. L. and Longas, M. O.: Structure-Function Relations of Heparin–Mimetic Sulfated Glucoronoxylyl-Oligosaccharides Isolated by a Combinatorial-Type Approach vs. Antiviral and Antithrombin Target Functions: Proton NMR Studies (1999), Glycobiology, 9, 10:1119.

12. Longas*, M. O., +Trinkle, J. A., +Schoeneck, C. D., Park, J. I. and Griep, J. A.: Dermatan Sulfate with Sulfated and Unsulfated Nonreducing Ends (1999), Glycobiology, 9, 10:1154.

13. Longas*, M. O., +Burden, J. D., +Booth, R. M. and +McPencow, J. A: Evidence for a Peptidase that N-Deacetylates Hyaluronic Acid (1998), Glycobiology, 8, 11:116.

14. Longas*, M. O., +Schoeneck, C. D. and +Oehlman, S. R.: Sulfated Hexosamine at the Nonreducing End of Dermatan Sulfate (1997), Glycobiology, 7, 7:1024.

15. Longas*, M. O. and Griep, J. A.: Uronic Acids, the Nonreducing Ends of Dermatan Sulfate (1995), Glycoconjugate J., 12, 4:485.

16. Longas*, M. O. and Griep, J. A.: Nonreducing Termini of Aging Dermatan Sulfate (1993), Glycoconjugate J., 10, 4:256.

17. Longas*, M. O. and Garg, H. G.: Typical Sulfate Composition of Burn Scar Dermatan Sulfate (1992), Glycobiology, 2, 5:492.

18. Longas*, M. O., Bhuyan, D. K. and Bhuyan, K. C.: Chemical Properties of Rat Skin Glycosaminoglycans (1991), Glycoconjugate J., 8, 3:199-200.

19. Longas*, M. O. and +Breitweiser, K. O.: Sulfate Composition of Glycosaminoglycans Determined by Infrared Spectroscopy (1990), Glycoconjugate J., 7, 5:509.

20. Longas*, M. O., Bhuyan, D. K., Bhuyan, K. C. and +Gutsch, C. M.: Effect of UV–Light Irradiation and Vitamin E Intake on Rat Skin Glycosaminoglycans (1988), Glycoconjugate J., 5, 5:523.

21. Koreen, R., Weinflash–Konstadt, J., Grabowski, G., Longas*, M. O., Toome, B., Lemlich, G. and Lebwohl, M. G.: Quantitative Evidence for Increased Hyaluronic Acid and Dermatan Sulfate in Lesional and Nonlesional Pseudoxanthoma Elasticum Skin (1987), J. Invest. Dermatol., 88, 4:500.

22. Weissman, D., *Perlish, J. S., Longas, M. O., Petrozzi, J. and Fleischmajer, R.: Growth, Collagen and Glycosaminoglycan Synthesis by Dermal Fibroblasts Derived from PUVA Treated and Psoriatic Patients (1987), J. Invest. Dermatol., 88, 4:523.

23. Longas, M. O.: Xylosyl-Alanine Is the Major Protein Linkage of Human Skin Dermatan Sulfate (1987), J. Invest. Dermatol., 88, 4:504.

24. Longas*, M. O., Azulay, D. R. and Fleischmajer, R.: Two Dermatan Sulfate Protein Linkages Involving Alanine, Serine and Xylose (1986), J. Invest. Dermatol, 86, 4:490.

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